– Design and develop more specialized instrumental analytical solutions and hardware optimizations as required to solve more complex bioanalysis projects. Examples include the use of multi-dimensional liquid chromatography in the more efficient extraction, purification, and separation of proteins and peptides; the use of more specialized liquid handling operations. The isolation and analysis of hydrophobic and in vivo modified proteins as they occur in biological specimens such as cells, tissues or biofluids (serum, plasma, CSF, ascetic fluids).
– Develop and implement methodological solutions to complex bioanalytical queries requiring independent analytical thinking and advanced knowledge.
– Contribute mass spectrometry expertise to basic and translational research and development to support on-going and perspective programs and collaborative projects.
– Contribute to the composition of relevant portions to manuscripts in preparation or conference presentations; prepare progress reports; maintain and refine standard operating procedures.
– Other duties as assigned.
– MSc. in Chemistry, Biochemistry, Pharmacology, or a related discipline.
– Detailed understanding of established proteomic assays and techniques, from basic bottom-up/top-down proteomics assays to more applications involving post translational modification analysis, proximity ligation, and chemical crosslinking.
– Willingness to take project ownership, and initiative in the design and development of novel bioanalytical methods.
– Ability to work well independently and in team settings.
– Ability to quickly assimilate new techniques and concepts.
– A working understanding of mass spectrometry fundamentals.
– Hands-on experience in the effective use of mainstream quantitative proteomics approaches, including both label free and isotopic labeling methods.
– Working expertise with robust LC/MS instrument tuning, calibration, and maintenance.
– Effective interpersonal communication skills to guide and support collaborators in addressing their experimental objectives.
– Willingness and ability to multi-task in a fast-paced and dynamic working environment.
– Solid foundation in the use of proteomic data curation, biostatistics, bioinformatics, and search algorithms.
– Ability to adopt new data analyses pipelines facilitated by various software tools such as R.
– Working expertise in absolute quantitative proteomic analysis using PRM/MRM/SRM LC/MS methods.
– Cover Letter
– Three references