MASS SPEC SERVICES: PROTEIN-IDENTIFICATION
We reliably and sensitively identify proteins from low-complex (e.g. single gel spots, purified protein etc.) as well as complex samples by our laboratory standardized identification workflow. In this, proteins are first proteolitically digested to peptides (gel-based or gel-free) and then analysed by LC-MS/MS using our highly sensitive Orbitrap-MS systems. Afterwards, these peptides/proteins are identified by using suitable data analysis pipelines (MS/MS Ion Search) and high confidence results are provided to the clients.
- Sample Preparation service
- Digestion (in-gel or in solution) service
- Accurate Molecular Mass determination of peptides or intact proteins
- Purity analysis of peptides or proteins
- Peptide mass fingerprinting service using nano-ESI LC MS/MS
- Membrane proteomics service
- Subcellular proteomics service
- Protein-protein interaction service
PROTEIN POST-TRANSLATIONAL MODIFICATION ANALYSIS
We perform the analyses of various posttranslational modifications (PTMs), for example phosphorylation, SUMOylation, etc. Please contact us for details and to discuss your specific project.
- Phosphorylation analysis
- Methylation analysis
- Acetylation analysis
- Ubiquitination analysis
- Nitrosylation analysis
- Di-Sulfide bond localization
- Customer-tailored service
QUANTITATIVE PROTEOMICS
WE OFFER SEVERAL QUANTIFICATION STRATEGIES:
Unbiased proteomic studies can be performed using our high resolution Orbitrap mass spectrometers to provide accurate quantification data. We provide Label-free methods for both relative and absolute quantification, which are rapid and a low-cost alternative to other quantitative proteomic approaches.
Label-free:
approaches to quantitative proteomics have gained prominence in recent years since no additional chemistry or sample preparation steps are required
SILAC:
stable isotope labeling of amino acids in cell culture,a global method whereby all translated proteins have isotope labels metabolically incorporated at selected amino acid residues;
DIA analysis
Building DDA spectral libraries, DIA aquisitions & Data analysis
PRM and SRM analysis for absolute quantification
Orbitrap based PRM analysis and Triple Quad technology based SRM/MRM analysis