ADVANCED METABOLOMICS SERVICES

DISCOVERY & TARGETED METABOLOMICS SERVICES

Service: VPMETA LC-MS/MS

LIST OF VPMETA SERVICES

Metabolomics is a powerful tool for studying metabolic processes, identifying crucial biomarkers responsible for metabolic characteristics and revealing metabolic mechanisms, which construct the content of discovery metabolomics. Discovery (nontargeted) metabolomics identify unknown metabolites in complex biological mixtures. The most common approach consists of searching available databases using exact mass and MS/MS spectral data.

Our facility high resolution, accurate mass (HRAM) mass spectrometers (MS)  provide extensive, rich, raw mass-spectral data that require sophisticated  tool-sets to turn them into useful insights about  system under investigation. This infrastructure has the ability to make retention time corrections, predict elemental formulas,  detect features, eliminate redundant data, annotate unknowns, find relevant  differences, and place information into its biological or process context.

Due to the inherent sensitivity of all mass spec equipment, it is imperative that only the highest quality plastics and reagents/solvents are used in the preparation and execution of any metabolomics based experiment. Plastic contaminants can be very misleading within a dataset and it is important that these are eradicated. All solvents used must be atleast LC-MS grade.

Metabolomics Compatible samples : Different protocols are available in literature for optimal extraction of metabolites from different experimental setup. Generally the extraction buffers used in metabolomics are organic solvents like methanol etc in combination with other solvents. The extraction buffer must be Mass Spec compatible and free from salts, glycerol and detergents.

We Generally receive lyophilized measured metabolite mixtures from our clients which we resuspend in MS compatible loading buffers and analyze in Mass Spec with buffer gradients as per the method requirements.

SERVICE CODETYPEMETHOD SPECIFICATIONS
VP-DEEPMETABO1DEEP DISCOVERY METABOLOMICSADVANCE ESI LC MS/MS ORBITRAP EXPLORIS, Routine Run Gradient
VP-DEEPMETABO2DEEP DISCOVERY METABOLOMICSADVANCE ESI LC MS/MS ORBITRAP FUSION, Routine Run Gradient

Sample to Structure:

PATHWAYS TO INSIGHT 

Know more unknowns

  • Identification of compounds using MS/MS libraries
  • Determination of elemental composition using high-resolution full MS isotopes and MS/MS data
  • Searching online chemical databases

Go from spectra to structure

  • Spectral library and similarity searches to identify compounds
  • Searching chemical databases for putative candidates
  • Annotation of spectra with predicted fragmentation, automatically

Find real differences

  • Statistics and differential analysis using t-tests, ANOVA, PCA, PLS-DA, volcano plots, box-and-whisker plots, and more
  • Trend charts to visualize peak areas, average peak area per group or fractional label incorporation over time
  • Generation of Heatmaps of peak areas, CV values, adjusted p-values

Service: VP-TAR-MET LC-MS/MS

Targeted metabolomics is an analytical application of mass spectrometry that encompasses the measurement of defined groups of chemically characterized and biochemically annotated metabolites. Targeted metabolomic platforms have become an integral part of clinical research and diagnosis, allowing the better characterization and understanding of pathophysiology mechanisms of disease, in particular for metabolic disorders.
Targeted metabolomics is quantitative and derives its information from discovery experiments, literature and/or clinical observations before a model or hypothesis is tested.

Testing involves verification and validation of defined groups of known metabolites across large sample sets. These experiments require accuracy, high throughput and reliability. For large scale targeted profiling, using a high resolution accurate mass (HRAM) workflow is optimal, while for routine quantitation, selected reaction monitoring (SRM) on a triple quadrupole MS is the preferred solution.

Triple Quandrupole Analysis

SERVICE CODETYPEMETHOD SPECIFICATIONS
VP-TAR METABO1TARGETED SRM METABOLOMICSADVANCE ESI LC MS/MS TRIPLE QUADRUPOLE MASS SPECTROMETER
VP-DEEPMETABO2TARGETED PRM METABOLOMICSADVANCE ESI LC MS/MS ORBITRAP MASS SPECTROMETER