MASS SPEC SERVICES: PROTEIN-IDENTIFICATION
We reliably and sensitively identify proteins from low-complex (e.g. single gel spots, purified protein etc.) as well as complex samples by our laboratory standardized identification workflow. In this, proteins are first proteolitically digested to peptides (gel-based or gel-free) and then analysed by LC-MS/MS using our highly sensitive Orbitrap-MS systems. Afterwards, these peptides/proteins are identified by using suitable data analysis pipelines (MS/MS Ion Search) and high confidence results are provided to the clients.
We perform the analyses of various posttranslational modifications (PTMs), for example phosphorylation, SUMOylation, etc. Please contact us for details and to discuss your specific project.
Unbiased proteomic studies can be performed using our high resolution Orbitrap mass spectrometers to provide accurate quantification data. We provide Label-free methods for both relative and absolute quantification, which are rapid and a low-cost alternative to other quantitative proteomic approaches.
approaches to quantitative proteomics have gained prominence in recent years since no additional chemistry or sample preparation steps are required
stable isotope labeling of amino acids in cell culture,a global method whereby all translated proteins have isotope labels metabolically incorporated at selected amino acid residues;
isotope-coded affinity tags, a technique that labels cysteine residues at the protein level
TMT & iTRAQ:
multiplexed isobaric tags for relative and absolute quantification
Building DDA spectral libraries, DIA aquisitions & Data analysis
PRM and SRM analysis for absolute quantification
Orbitrap based PRM analysis and Triple Quad technology based SRM/MRM analysis
K-37A, Ground Floor Green Park Main, New Delhi 110016
Phone: +91-11-4050 2621