TECHNOLOGY

MASS SPEC SERVICES: PROTEIN-IDENTIFICATION

We reliably and sensitively identify proteins from low-complex (e.g. single gel spots, purified protein etc.) as well as complex samples by our laboratory standardized identification workflow. In this, proteins are first proteolitically digested to peptides (gel-based or gel-free) and then analysed by LC-MS/MS using our highly sensitive Orbitrap-MS systems. Afterwards, these peptides/proteins are identified by using suitable data analysis pipelines (MS/MS Ion Search) and high confidence results are provided to the clients.

  • Sample Preparation service
  • Digestion (in-gel or in solution) service
  • Accurate Molecular Mass determination of peptides or intact proteins
  • Purity analysis of peptides or proteins
  • Peptide mass fingerprinting service using nano-ESI LC MS/MS
  • Membrane proteomics service
  • Subcellular proteomics service
  • Protein-protein interaction service

 

PROTEIN POST-TRANSLATIONAL MODIFICATION ANALYSIS

We perform the analyses of various posttranslational modifications (PTMs), for example phosphorylation, SUMOylation, etc. Please contact us for details and to discuss your specific project.

  • Phosphorylation analysis
  • Methylation analysis
  • Acetylation analysis
  • Ubiquitination analysis
  • Nitrosylation analysis
  • Di-Sulfide bond localization
  • Customer-tailored service

 

QUANTITATIVE PROTEOMICS

We offer several quantification strategies:  

Unbiased proteomic studies can be performed using our high resolution Orbitrap mass spectrometers to provide accurate quantification data. We provide Label-free methods for both relative and absolute quantification, which are rapid and a low-cost alternative to other quantitative proteomic approaches.

Label-free:

approaches to quantitative proteomics have gained prominence in recent years since no additional chemistry or sample preparation steps are required

SILAC:

stable isotope labeling of amino acids in cell culture,a global method whereby all translated proteins have isotope labels metabolically incorporated at selected amino acid residues;

ICAT:

isotope-coded affinity tags, a technique that labels cysteine residues at the protein level

TMT & iTRAQ:

multiplexed isobaric tags for relative and absolute quantification

DIA analysis

Building DDA spectral libraries, DIA aquisitions & Data analysis

PRM and SRM analysis for absolute quantification

Orbitrap based PRM analysis and Triple Quad technology based SRM/MRM analysis

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VProteomics

K-37A, Ground Floor
Green Park Main,
New Delhi 110016
india

Phone: +91-11-4050 2621

Email: info@vproteomics.com

Website: www.vproteomics.com

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